BKS DB Mouse Spleen Endothelial Cells -1 vial

Species : BKS DB Mouse Description: BKS db Mouse Spleen Endothelial Cells are isolated from the spleen of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic BKS db Mouse Spleen Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in AcceGen’s Culture Complete Growth Medium generally for 3-7 days Cultures are then expanded Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials Each vial contains at least 05×106 cells per ml The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibodyand directly supplied to your lab by Gentaur's warehouse.