Rat Pulmonary Artery Fibroblasts -1 vial

Species : Rat Description: Rat Primary Pulmonary Artery Fibroblasts from AcceGen are isolated from tissue of neonatal Sprague-Dawley Rats Rat Primary Pulmonary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 05 hour and incubated in AcceGen’s Cell Culture Medium for 3-7 days Cultures are then expanded Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials Each vial contains at least 1×10^6 cells per ml and is delivered frozenCells are negative for bacteria, yeast, fungi, and mycoplasma Rat Primary Pulmonary Artery Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen Repeated freezing and thawing of cells is not recommendedStandard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applicationsand directly supplied to your lab by Gentaur's warehouse.