Rat Ovarian Epithelial Cells -1 vial

Species : Rat Description: Rat Ovarian Epithelial Cells from AcceGen are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats Rat Ovarian Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 05 hour and incubated in AcceGen’s Cell Culture Medium for 3-5 days Cells are detached from flasks and immediately cryo-preserved in vials Each vial contains 1×10^6 cells and is delivered frozenRat Ovarian Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1 Rat Ovarian Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by AcceGen Repeated freezing and thawing of cells is not recommendedStandard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applicationsand directly supplied to your lab by Gentaur's warehouse.