Rat Colonic Smooth Muscle Cells -1 vial

Species : Rat Description: Rat Colonic Smooth Muscle Cells from AcceGen are isolated from tissue of Sprague-Dawley Rats Rat Colonic Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 05 hour and incubated in AcceGen’s Cell Culture Medium for 3-7 days Cultures are then expanded Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials Each vial contains at least 1×10^6 cells per ml and is delivered frozenCells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actinCells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGenRepeated freezing and thawing of cells is not recommendedStandard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applicationsand directly supplied to your lab by Gentaur's warehouse.