CD-1 Mouse Lung Mesenchymal Stem Cells -1 vial

Species : CD-1 Mouse Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult mice Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 05 hour and incubated in AcceGen’s Cell Culture Medium for 7-15 days Cultures are then expanded Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials Each vial contains at least 1×10^6 cells per ml and is delivered frozenMouse Lung Mesenchymal StemCells are negative for bacteria, yeast, fungi, and mycoplasma Cells can be expanded 3-8 passages by the ratio of 1:3 under the cell culture conditions specified by AcceGen Repeated freezing and thawing of cells is not recommendedMouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 positive by flow cytometryStandard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applicationsand directly supplied to your lab by Gentaur's warehouse.