C57BL/6 Mouse Pulmonary Artery Fibroblasts -1 vial

Species : C57BL/6 Mouse Description: C57BL/6 Mouse Primary Pulmonary Artery Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice Mouse Primary Pulmonary Artery Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 05 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days Cultures are then expanded Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials Each vial contains at least 1×10^6 cells per ml and is delivered frozenMouse Primary Pulmonary Artery Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining Cells can be expanded for 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen Repeated freezing and thawing of cells is not recommendedStandard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applicationsand directly supplied to your lab by Gentaur's warehouse.