C57BL/6 Mouse Bone Marrow Macrophages -1 vial

Species : C57BL/6 Mouse Description: Mouse Bone Marrow Macrophages are derived from the tibias and femurs of pathogen-free laboratory adult mice Mouse Bone Marrow Macrophages are grown in 100 mm treated tissue culture dish and incubated in AcceGen’s Culture Medium for 7-10 days Cultures are then expanded Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials Each vial contains at least 6×10^6 cells and is delivered frozenMouse Bone Marrow Macrophages are negative for bacteria, yeast, fungi, and mycoplasma Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen Repeated freezing and thawing of cells is not recommendedMouse Bone Marrow Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometryStandard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applicationsand directly supplied to your lab by Gentaur's warehouse.